Thaw the ladder either at room temperature or at 37-40☌ for a few minutes to dissolve precipitated solids. VisColor Pre-Stained Protein Marker is a ready-to-use protein standard with 10 pre-stained. The quality of the BlueRAY Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality.īlueRAY Prestained Protein Ladder Protocolġ. Multi-color protein ladder for SDS-PAGE and Western blotting. Sizing of proteins on SDS-PAGE gels and Western blots.Īpproximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25☌), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol. Monitoring of protein transfer onto membranes during Western blots. Monitoring of protein migration during SDS-PAGE. Ready-to-use: supplied in a loading buffer for direct loading on gelsĮasy to identify: includes the ~25, ~75 kDa reference bands coupled with a green and a red dye Do not heats, dilute, and add reducing agent before loading.īroad range: 10-180 kDa (Tris-glycine-SDS running buffer) The ladder is supplied in gel loading buffer and is ready to use. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode. Compare and view all other protein standards and ladders The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated during SDS-PAGE, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximate sizing of proteins. Prestained Protein Marker with increased brightness provides robust visualization for western blotting workflow, identifying target proteins on the gel or. 5 l of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with Tris-glycine-SDS running buffer. The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kilodalton (kDa).
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